EFFECT OF BISPHENOL A ON THE MORPHOFUNCTIONAL CHARACTERISTICS OF EJACULATE
DOI:
https://doi.org/10.32689/2663-0672-2023-4-11Keywords:
bisphenol A, еlectroejaculation, spermatozoa, spermatogenesis, fertilityAbstract
Introduction. Studies of the pathological effect of pollutants on the processes of spermatogenesis indicate the most likely causes of male infertility. The main reasons include the influence of endocrine disruptors and the most common component of industrial plastic products – bisphenol A. It is noted that the most important aspects of male fertility are the quality of sperm. The mechanisms of this effect, especially with prolonged exposure to bisphenol A, are currently poorly understood, which makes it important to study this medical problem. The aim of the study was to conduct a comparative analysis of the long-term effect of bisphenol A (BPA) on rat ejaculate quality as a fundamental biomarker of reproductive function. Materials and methods. The study was conducted on 120 white male rats of the Wistar line. The animals were randomly divided into four groups: control group, comparison group – the animals were injected with corn oil; in the third and fourth groups, the rats were injected with BPA dissolved in corn oil, respectively, at doses of 50 and 250 mg/kg/day. The result. It was found that long – term use of BPA, even in small doses, caused negative changes in the studied parameters of spermatozoa-the number, motility and morphology of spermatozoa, which indicates a violation in the rat spermatogenesis system. A decrease in the total sperm count was determined by 21.8%, P<0.05, and 32.4%, P<0.05 in groups F0-3 and F0-4 under the influence of BPA. The proportion of progressively motile spermatozoa had a steady downward trend, and at the end of the experiment, the parameter value was 22.7%, P<0.05 and 37.4%, and P<0.05 in groups F0-3 and F0-4, respectively. Prolonged exposure to bisphenol A resulted in a statistically significant increase of more than 2.7 times, P<0.05, in the number of Immobile spermatozoa in animals of groups F0-3 and more than 5.7 times, P<0.05, in Group F0-4 compared to the control group. Administration of ARBs at a dose of 250 mg/kg/day resulted in statistically significant changes in the proportion of abnormal sperm forms compared to the control group. Conclusion. Under the influence of BPA, complexes of disorders were gradually formed that changed the qualitative and quantitative characteristics of rat spermatozoa: a progressive decrease in the total number of spermatozoa in the ejaculate aliquot, a decrease in the number of germ cells of the fertile fraction, and an increase in the number of cells of the non-fertile fraction.- fertile fraction, as well as reduced mobility of germ cells.
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