PATHOPHY SIOLOGICAL MECHANISMS OF COLL AGEN MATRIX DESTABILIZ ATION IN THE DESCENDENTS OF WISTAR RATS IRRADI ATED WITH DI FFERENT DOSES
DOI:
https://doi.org/10.32689/2663-0672-2025-4-3Keywords:
Ionizing radiation, connective tissue, offspring of irradiated rats, hydroxyproline, vitamin C, collagenolysis, collagen matrix, biomarkers, pathophysiological mechanismsAbstract
The aim of the study is to investigate the pathophysiological mechanisms of collagen matrix destabilization in the offspring of rats irradiated with different doses of ionizing radiation (IR), as well as to substantiate their use as early biomarkers for predicting connective tissue disorders. Methodology. Biochemical methods were used to determine the levels of hydroxyproline and ascorbic acid in the biological fluids (serum, urine) of the rat offspring. Changes in the metabolism of hydroxyproline were assessed depending on the doses of parental irradiation (1.0 Gy, 3.0 Gy, 5.82 Gy), as well as the levels of ascorbic acid in serum and urine. Scientific novelty. Dose-dependent changes in the metabolism of hydroxyproline and vitamin C levels in the biological fluids of the offspring of irradiated animals were demonstrated. New data were obtained regarding the use of hydroxyproline fractions (free, peptide-bound, protein-bound) and the level of ascorbic acid as sensitive biomarkers for early detection of collagen matrix destabilization. Conclusions. The study showed that irradiation of parental animals with different doses of ionizing radiation leads to significant changes in the metabolism of hydroxyproline and a decrease in the level of ascorbic acid in the biological fluids of the offspring. These changes are indicators of collagenolysis activation and antioxidant system depletion. Hydroxyproline and vitamin C can be used as early, sensitive biomarkers for monitoring radiation-induced connective tissue disorders, which is important for predicting and treating structural and functional disorders.
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