COMPARATIVE CHARACTERISTICS OF MORPHOMETRIC PARAMETRS OF SINUSOIDAL HEMOCAPILLARIES OF THE LIVER AFTER 2 AND 4 WEEKS OF EXPERIMENTAL EXPOSURE TO 10% CANNABIDIOL OIL

Abstract

Introduction. Cannabidiol (CBD) is a non-psychoactive phytocannabinoid found in Cannabis sativa, the popularity, use and availability of which is growing worldwide. CBD has been shown to be therapeutically effective in treating severe epileptic diseases in children, and is also used to treat pain, anxiety, and depression, among other things. However, there is evidence in the literature of side effects and hepatotoxicity associated with the use of CBD. Additional experimental and clinical studies are needed to determine the nature and severity of liver damage, including vascular damage, and hemodynamic features, particularly at the level of the capillary network. The purpose of the study: to determine the features of the morphological organization and morphometric parameters of sinusoidal hemocapillaries of the liver after 2 and 4 weeks of experimental exposure to 10% Cannabidiol oil. Methodology: The material for morphological research was the liver tissue of rats of the control and experimental groups. The experimental group was divided into 2 series of rats with different terms of exposure to 10% cannabidiol oil. Rats of the first series (14 individuals) were given orally dripped 10% cannabidiol oil at a dose of 10 mg/kg/day once a day for 2 weeks, rats of the second series (14 individuals) were given orally dripped 10% cannabidiol oil at a dose of 10 mg/kg/day once a day for 4 weeks. The experiments were conducted in accordance with the provisions of the European Convention for the Protection of Vertebrate Animals Used for Experimental and Other Scientific Purposes (Strasbourg, 1986), Council of Europe Directive 2010/63/EU, Law of Ukraine No. 3447-IV “On the Protection of Animals from Cruelty to Animals”. To objectify the results of the study, a morphometric method was used to determine the diameters of sinusoidal hemocapillaries of the liver of rats of the control and experimental groups of both series using the Aperio ImageScope v12.3.3 software (Leica biosystems, Wetzlar, Germany). Statistical calculations were performed. The difference was considered statistically significant at a minimum significance level of p<0.05. Scientific novelty. The microscopic organization of the liver lobule was preserved and did not undergo histopathological changes under the conditions of experimental exposure to 10% CBD oil (10 mg/kg/day) as a dietary supplement for two and four weeks. The average diameter of the sinusoids in both series of the experimental group did not differ significantly from the control (p>0.05) and did not differ in dynamics (p>0.05). Diagnosed single dilated and hyperemic sinusoids in two series of the experimental group, respectively in 21.43% and 28.57% did not differ from the control group (20%) (p>0.05). Conclusions. The results of morphological and morphometric analysis of liver sinusoids after two and four weeks of experimental exposure to CBD indicate the safety of using a 10% CBD oil at a dose of 10 mg/kg/day.