ANTIOXIDANT ACTIVITIES OF PETROLEUM ETHER, ETHYL ACETATE, N-BUTANOL AND AQUEOUS EXTRACTS FROM SPERANSKIA TUBERCULATA (BUNGE) BAILL HERB

Abstract

The article examines the antioxidant properties of various extracts of the herb Speranskia Tuberculata (Bunge) Baill. Crude extracts were obtained using petroleum ether, ethyl acetate, n-butanol and water. Subsequently, the dried extracts were dissolved in dimethylsulfoxide to prepare working solutions with 10 to 100 μg/mL concentrations. The work aims to study the antioxidant effect of different concentrations of Speranskia Tuberculata (Bunge) Baill herb extracts obtained by dissolving in 4 solvents. Methods. The antioxidant activity of the extracts was evaluated, and total concentrations of phenolic compounds and flavonoids were determined. Results. N-butanol extracts demonstrated the lowest antioxidant activity, while the highest activity was characteristic of ethyl acetate and petroleum ether extracts. Using experimental data, IC50 was calculated for all solvents and both measurement methods. For the DPPH radical scavenging rate method, the IC50 ranged from 74 (n-butanol) to 35 μg/mL (petroleum ether). For the superoxide radical anion absorption rate method, the IC50 ranged from 41 (n-butanol) to 20 μg/mL (ethyl acetate). The determined concentrations of phenolic compounds varied from 280 (ethyl acetate) to 7 (petroleum ether) mg/GE/g. For flavonoids, the range was 167 (ethyl acetate) – 7 (petroleum ether) mg/RE/g. The obtained results made it possible to classify the studied extracts with high antioxidant activity, except for the n-butanol solution with an average activity level according to the DPPH radical absorption rate method results. Conclusions. Ethyl acetate extracts showed the highest total content of phenolic compounds and flavonoids, and ethyl acetate and petroleum ether extracts showed the highest antioxidant activity.