DETECTION OF ANTIMICROBIAL EFFECT OF NEW COMPOSITIONS WITH DIINDOLILMETHANE ON FORMED MICROBIAL BIOFILMS

Abstract

Introdction. Antimicrobial resistance is a global threat to public health, which permeates all domains of modern medicine, the use of drug combinations to re-sensitize resistant strains has emerged as a promising means to bypass the stagnant drug discovery pipeline. The aim of the study was to determine the antimicrobial effect of compositions based on 3,3’-diindolylmethane (DIM) with antimicrobial drugs in vitro on daily biofilms of reference and clinical, multi-resistant strains of microorganisms. Materials and methods. Identification of microorganisms was carried out using the «MIKROLATEST®» kits. The sensitivity of clinical strains to antimicrobial drugs was studied using a microtest system with semi-quantitative registration of results «MIKROLATEST SensiLaTest MIC» (ERBA, Lahema). Analysis of the results was provided using an automatic analyzer «Multiskan EX» (type 355). Research results and their discussion. It was found that the experimental compositions based on DIM with ofloxacin or dioxidine were effective against reference and clinical strains. The introduction of these experimental compositions into bacterial biofilms led to damage of its integrity and formation of morphological differences from the control daily biofilms. Visualization of biofilms in 24 hours after the addition of ofloxacin or dioxidine showed the formation of a biofilm almost similar to the control one, but partial destruction of its integrity was recorded. Thus, when using the experimental composition «ofloxacin + DIM + soluble base» the proportion of cells with a damaged membrane was 69.4%, and when adding the experimental sample «dioxidin + DIM + soluble base» the proportion of cells with a damaged membrane was 61.8%. Adding samples containing «DIM + soluble base» and only «soluble base» without antimicrobial drugs caused partial destruction of the daily biofilm. Conclusions. Investigating the effect of DIM in new compositions with antimicrobials, which are most often used in the treatment of purulent-inflammatory diseases, on dense biofilms formed by resistant strains, which are the cause of lethal consequences, it was found that compositions with DIM are able to affect the biofilms formed by microorganisms by destroying them. Thus, DIM has properties that inhibit the process of biofilm formation and destroy already formed dense biofilms.