PECULIARITIES OF RELATIONSHIPS BETWEEN CLINICAL, HYGIENIC AND MICROBIOLOGICAL PARAMETERS USED FOR THE PREDICTION OF CARIES INTENSITY LEVELS AMONG MONOZYGOTIC TWINS AGED 18–25 YEARS
DOI:
https://doi.org/10.32689/2663-0672-2023-3-6Keywords:
caries, monozygotic twins, oral microflora, caries predictionAbstract
Introduction. There is still a lack of data in the literature regarding characteristics of caries progression among adult twins, since most of the studies available for analysis are focused on monitoring pediatric pateints, or until they reach adulthood. In addition, some publications demostrate trend towards an increase of the variability levels of carious pathology among monozygotic twins parallel with an increase in the average age indicator, and the dominance of various clinical and microbiological indicators as potential predictors for caries development, but not the genetic component. Objective. To determine the significance of clinical, hygienic and microbiological parameters, as well as their combination for predicting caries intensity levels among monozygotic twins aged 18–25 years. Methods. Clinical, hygienic and microbiological parameters of the oral cavity of 23 pairs of monozygotic twins (14 male and 9 female) aged 18–25 years were evaluated during the study. During the clinical examination patients were evaluated for the level of enamel resistance according to the TER-test methodology, the state of oral hygiene according to the OHI-S index, as well as the intensity of caries according to the DFM index. During the patient questionnaire, the frequency of brushing teeth during the day, the number of visits to the dentist for preventive purposes during the last year, as well as the average number of meals during the day were determined. The results of the quantitative and qualitative evaluation of the microbiota of the oral cavity were expressed in CFU/ml. Results. The average DFM indicator among monozygotic twins aged 18–25 was 7,39±0,44. Caries intensity values were most correlated with indicators of the established concentration of S. salivarius (r=0,741, p < 0,0001), indicator of OHI-S (r=0,709, p < 0,0001), concentration of S. mutans (r= 0,698, p < 0,0001) and TER-test indicator (r=0,672, p < 0,0001), while correlations with the frequency of brushing teeth during the day and the number of meals during the day were characterized by lower levels of correlation (r=- 0,058 and r=-0,0652, p < 0,05); correlation with the frequency of visits to the dentist during the year did not confirm its statistical significance (r=-0,0114, p=0,563). Conclusions. 54,9% and 48,8% of the variability of caries intensity in the sample of monozygotic indicators aged 18–25 years can be predicted based on the analysis of changes in the concentration of S. salivarius and S. mutans as explanatory variables. In general, the use of indicators of the TER test, the OHI-S index, the frequency of brushing teeth, the frequency of visits to the dentist during the year for preventive purposes, the number of meals during the day, the concentration of S. mutans and S. salivarius as regressors made it possible to achieve the appropriateness of the caries intensity prediction model at the level of R2=0,805 and adjusted R2=0,737 taking into account the different significance of the studied factors’ impact.
References
Genetic factors affecting dental caries risk / S. Opal, S. Garg, J. Jain, [et al.]. Australian dental journal. 2015. Vol. 60(1). P. 2–11.
Genetic and early-life environmental influences on dental caries risk: a twin study / M.J. Silva, N.M. Kilpatrick, J.M. Craig, [et al.]. Pediatrics. 2019. Vol. 143(5). P. e20183499
Cogulu D., Saglam C. Genetic aspects of dental caries. Frontiers in Dental Medicine. 2022. Vol. 3. P. 1060177.
Heritability of caries scores, trajectories, and disease subtypes / S. Haworth, A. Esberg, P. Lif Holgerson, [et al.]. Journal of Dental Research. 2020. Vol. 99(3). P. 264–270.
Deshpande A., Deshpande N. Similar caries pattern in monozygotic twins: Role of nature and/or nurture. European Journal of General Dentistry. 2021. Vol. 1(02). P. 104–108.
Pyrosequencing of plaque microflora in twin children with discordant caries phenotypes / M. Zhang, Y. Chen, L. Xie, [et al.]. PLoS One. 2015. Vol. 10(11). P. e0141310.
Research on oral microbiota of monozygotic twins with discordant caries experience-in vitro and in vivo study / H. Wu, B. Zeng, B. Li [et al.]. Scientific Reports. 2018. Vol. 8(1). P. 7267.
Silva M. J., Kurushima Y., Scurrah K. Twin and family studies on epigenetics and oral health. Twin and Family Studies of Epigenetics. 2021. Vol. 27. P. 85–104.
Evaluation of Inter-pair Differences in Caries among South Indian Monozygotic Twins: A Cross-sectional Study / R. Zarina, S. Kuriakose, J. Lalithamma, [et al.]. International Journal of Clinical Pediatric Dentistry. 2019. Vol. 12(4). P. 318.
Consortium-based genome-wide meta-analysis for childhood dental caries traits / S. Haworth, D. Shungin, J.T. Van Der Tas, [et al.]. Human molecular genetics. 2018. Vol. 27(17). P. 3113–3127.
Rintakoski K., Kaprio J., Murtomaa H. Genetic and environmental factors in oral health among twins. Journal of dental research. 2010. Vol. 89(7). P. 700–704.
Longitudinal study of oral microbiome variation in twins / M. Freire, A. Moustafa, D.M. Harkins [et al]. Scientific reports. 2020. Vol. 10(1). P. 7954.
Maslarski I. Qualitative dermatoglyphic traits in twins. Acta Morphologica et Anthropologica. 2015. Vol. 22. P. 75–87.
Maslarski I., Yaneva G. Dermatoglyphic methodology for establishing zygosity in the twins. J of IMAB. 2020. Vol. 26(3). P. 3313–3316.
Danyltsiv L. O., Rozhko, M. M. Study of structural and functional acid resistance of permanent tooth enamel in children depending on the characteristics of different level of anxiety. Art of Medicine. 2022. Vol. 22(2). P. 32–36.
Association between saliva quantity and content parameters with caries intensity levels: A cross-sectional study among subcarpathian children / L. Bilyschuk, A. Keniuk, M. Goncharuk-Khomyn, [et al.]. Pesquisa Brasileira em Odontopediatria e Clinica Integrada. 2019. Vol. 19(1). P. e5048–e5048.
Analysis of Environmental and Person-Oriented Factors Influence on Dental Caries Intensity among Children Population of Transcarpathia / M.O. Fera, O.V. Fera, V.M. Kryvanych, [et al.]. Journal of International Dental and Medical Research. 2020. Vol. 13(4). P. 1326–1333.
Pratten J., Wilson M., Spratt, D. A. Characterization of in vitro oral bacterial biofilms by traditional and molecular methods. Oral microbiology and immunology. 2003. Vol. 18(1). P. 45–49.
Isolation and identification of oral bacteria and characterization for bacteriocin production and antimicrobial sensitivity / M. Rahman, M.N. Islam, M.N. Islam, [et al.]. Dhaka University Journal of Pharmaceutical Sciences. 2015. Vol. 14(1). P. 103–109.
Oral microbiota and dental caries data from monozygotic and dizygotic twin children / Y, Kasimoglu, M. Koruyucu, S. Birant, [et al.]. Scientific Data. 2020. Vol. 7(1). P. 348.
Clinical assessment of deleterious oral habits and dental caries–periodontal parameters among Turkish twins / S.C. İlisulu, S. Birant, Y. Kasimoglu, [et al.]. Journal of Dental Sciences. 2023. Vol. 18(4). P. 1859–1866
Subramaniam P., Babu K. G., Vardhana B. Assessment of dental caries and oral hygiene status among twins. Journal of Forensic Science and Medicine. 2018. Vol. 4(1). P. 18–22.
