THE EFFECT OF THE DEVELOPED THERAPEUTIC AND PREVENTIVE COMPLEXES ON THE BIOCHEMICAL INDICES OF THE PULP OF EXPERIMENTAL ANIMALS WITH TEETHING DISORDER AGAINST THE BACKGROUND OF A CARIOGENIC DIET
DOI:
https://doi.org/10.32689/2663-0672-2024-1-9Keywords:
experiment, teething, dental pulp, biochemical indicesAbstract
Introduction. Teething disorder is a common problem in dentistry, which can contribute to the occurrence of diseases of the hard tissues of the teeth, cause inflammatory processes of periodontal tissues and lead to orthodontic pathology. Objective: to study the effect of the developed therapeutic-preventive complexes on the activity of lysozyme, acid and alkaline phosphatase of the dental pulp in rats with teething disorder against the background of a cariogenic diet. Materials and methods. The 1st (control) group consisted of 8 intact rats. The 2nd group consisted of 10 rats with early teething (born to females receiving L-thyroxine during pregnancy and lactation). The 3rd group comprised 10 rats with early teething (born to females receiving L-thyroxine during pregnancy and lactation) who received a therapeutic and preventive complex (TPC) No1. The 4th group consisted of 10 rats with a delayed teething (born to females receiving antibiotics during pregnancy and lactation). The 5th group included 11 rats with delayed teething (born to females receiving antibiotics during pregnancy and lactation) who received TPC No 2. The 6th group covered rats, which were modeled the delay in teething (born to females who received Mercazolil during pregnancy and lactation). The 7th group consisted of rats with delayed teething (born to females receiving Mercazolil during pregnancy and lactation) who received TPC No 2. Calcite gel was applied to the teeth of animals receiving TPC No. 1 within 20 days. During the next 20 days, the rats were given applications of Biotrite Dent gel on their teeth with simultaneous oral use of Biotrite Dent (400 mg/kg). After that, three times a day, the teeth of the rats were treated with the deep fluoridation system Ftorcalcit-E. For 30 days, the rats receiving TPC No. 2 were applied with Calcite gel on their teeth, and the rats received Mineralol (1g/kg) orally. During the next 30 days, applications of Biotrite Dent gel were applied to the teeth with simultaneous oral use of Biotrite Dent (400 mg/kg). During the entire experiment, the rats were orally treated with Lactiale Germina Forte (2 ml/kg). Acid phosphatase (ACP), alkaline phosphatase (ALP) and lysozyme activity was determined in the pulp of rat incisors. Results. The animals of the 3rd group with early teething, which were treated with TPC No. 1, showed lower activity of acid phosphatase (ACP) by 54.93%, and the activity of alkaline phosphatase (ALP) was higher by 53.38% than in the 2nd group of animals. The animals of the 5th group with delayed teething, which were treated with TPC No. 2, the activity of ACP was lower by 55.89%, and the activity of ALP was 2.08 times higher than in the 4th group of animals. The use of TPC No. 1 in animals of the 3rd group allowed to increase the rate of lysozyme in the pulp by 9.57 times compared to that in rats of the 2nd group. Conclusions. The results of the study indicate an improvement in the mineralization processes of the hard tissues of the teeth and antimicrobial protection in the pulp of the teeth in rats that were treated with the developed TPC.
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